REAL-TIME MONITORING OF RHAMNOSE INDUCTION EFFECT ON THE EXPRESSION OF MPT64 GENE FUSED WITH PELB SIGNAL PEPTIDE IN ESCHERICHIA COLI BL21 (DE3)

Real-time monitoring of rhamnose induction effect on the expression of mpt64 gene fused with pelB signal peptide in Escherichia coli BL21 (DE3)

HARMONIZED PROTEIN In this research, Escherichia coli BL21 (DE3) harboring an expression vector constructed with a rhamnose-inducible promoter and a pelB signal peptide was used as a host cell to produce MPT64 protein.The objective of this research was to figure out the optimum time of mpt64 gene expression through real-time monitoring of MPT64 pro

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Biallelic inheritance in a single Pakistani family with intellectual disability implicates new candidate gene RDH14

Abstract In a multi-branch family from Pakistan, individuals presenting with palmoplantar keratoderma segregate in autosomal dominant fashion, and individuals with intellectual disability (ID) segregate in apparent autosomal recessive fashion.Initial attempts to identify the ID locus using homozygosity-by-descent (HBD) mapping were unsuccessful.How

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